Microaerobic workstation; 4-gas system with built-in gas sensing technology

Microaerobic workstation; 4-gas system with built-in gas sensing technology

Microaerobic Workstation

The M135 HEPA is a tall, wide, deep microaerobic chamber. This variable atmosphere workstation is ideal for the study and isolation of fastidious microaerophiles where HEPA filtration is required. It has an internal volume of 900 litres and can accommodate a variety of items of equipment such as imaging devices, microscopes, plate readers, spiral platers, etc.

The generous internal height facilitates easy pipetting. All your work can be performed without removing your samples from the required microaerobic conditions. The entire chamber is a working and incubation space.

A rapid 16 liter airlock – no risk of compromising conditions inside the chamber (and it’s flushed with nitrogen as an additional cost saving).

This is a 4-gas system with built in oxygen, CO2 and hydrogen sensors allowing users to program precise gas concentrations, perfect for manipulating samples in a sustainable microaerobic environment. These workstations provide the most flexibility of any models in the Whitley Workstation range; allowing Anoxic/Anaerobic, Microaerobic or Hypoxic atmospheres to be created if desired without the need to change incoming gasses.

The M135 HEPA is fitted with the unique Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly.


Two patented oval ports enable you to work comfortably, gloved or bare handed.
Rather than use off-the-shelf HEPA filters, our manufacturer, DWS, has worked with one of the world's leading filter manufacturers to develop a filter specifically tailored to suit this workstation.
Levels of atmospheric cleanliness inside the workstation exceeds the requirements of ISO 14644 Class 3 (equivalent to Class 1 of the US Fed Std 209E Cleanroom Standards and exceeds the particle counts attained at rest’ (without an operator) of Grade A as set out in the GMP Guidelines). We can provide test results confirming particulate levels inside the workstation.
Fitted with a fan that circulates the internal atmosphere continuously - contributing to even temperature distribution and ensuring any internal particulate will be in motion.
A removable front enables the transfer of larger batches of samples or pieces of equipment for use inside the chamber.
Color, touch screen control panel for ease of use and for visual display of parameters such as temperature, humidity and airlock cycle status.


HEPA Fitted as standard
Capacity (Liters) 900.00
Gas Supplies H2 + N2 / CO2 / N2 / Air
Port Airlock Capacity 10 x 500ml Duran bottles or 40 plates
Conventional Porthole System Fitted as standard
Porthole system Manual
Airlock Cycle Time 60 seconds
Airlock size liters 16
Automatic Dehumidifier Fitted as standard
Removable Front Option Available
Temperature range 4ºC above ambient up to 45ºC
Dimensions (w/d/h - mm) 1452 x 1054 x 993
Weight (lbs/kg) 382 / 175


Rotating Turntable
A rotating turntable (up to 3 per shelf) to hold up to 80 x 90mm Petri dishes

Platform Assbemly
In sizes 440 x 400mm and 520 x 520mm robust platform with castors, designed to help move larger equipment around inside a Whitley Workstation

Double Internal
Power Socket
Facilitate the use of electrical equipment inside the chamber.

Internal Storage Trays
Maximum 2 per unit; positioned under the shelf.

Whitley Enhanced
Biological Containment
Some Whitley Workstation users require additional safeguards to reduce the possibility of bacteria contained inside workstations being released via the main chamber exhaust valve and the airlock exhaust valve. To address this requirement our Whitley HEPA workstations can be specified with additional HEPA filters fitted to these exhaust valves.

Vacuum Take-off Port
Provides a method of aspirating used liquid media from inside the workstation in safety and without the risk of spillage or contamination.

Single Plate
Entry System
Front loading Single Plate Entry System (or Letterbox)

Cable Gland
Cable glands allow cables and probes to be introduced into a Whitley Workstation without compromising internal conditions. A small cable gland is available with a clamping range between 3 - 7mm in diameter.

Multiple Cable Port
Unique to DWS, this option allows various cables and probes to be introduced into the chamber without compromising internal conditions. The port is 50mm in diameter.

Our custom-designed trolley frees up bench space and allows the chamber to be moved. The trolleys are built with high quality tubular steel and smooth rolling lockable wheels. They offer a sturdy and stable frame to support your use of our anaerobic and Microaerobic workstations.
See Details

Gas Pressure Monitor
Designed as a method of monitoring the gas supply. The system will indicate the status of both sides of the switchover: FULL – IN SERVICE, and DEPLETED. When a cylinder or cylinder bank is depleted, the green light will change to a red light, and the audible alarm will sound.
See Details

Temperature Mapping
Data is collected from up to 12 sensors, traceable to national standards, positioned throughout the workstation chamber. This is preferably a factory-based test but it can be performed on-site if necessary.

15cm Side
Entry Letterbox
Ideal for quickly introducing small quanitites of samples and supplies

Large Cable Gland
A large cable gland with a clamping range of 18 – 25 mm in diameter is available.

Data Logging
Access temperature, humidity, chamber pressure and anaerobic conditions. Stores up to 30 days of system information, accessed via USB port.

Data Logging with
Oxygen Profiling
This option allows the user to record the data from the environmental parameters inside the workstation AND allows the user to pre-program different oxygen levels.

Electronic Gas
Leak Detector
Provides an audible tick and flashing LED when exposed to H2 gas at 50 ppm or more. This device also has a tip light to illuminate dark areas.

Internal Storage Trays
Maximum 2 per unit; positioned under the shelf

Whitley Transfer
Connect an i2 workstation to selected Whitley Workstations. Transfer Cell Plates from the connected workstation without subjecting cells to ambient laboratory conditions.

Front Loading
9cm Letterbox
A single sample entry system is ideal for quickly introducing culture flasks, Petri dishes and other small items. This 9cm front loading 'letterbox' is retrofittable if the unit has a removable front.

Single Point
This is essential when a laboratory must demonstrate that temperature performance could directly or indirectly affect results. It provides independent, UKAS-accredited testing and supporting certification. It also confirms, through measurements taken from a single position within the workstation incubation area, using a calibrated temperature probe traceable to national standards, that the workstation temperature is controlled and displayed within specified tolerances.


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Removable Front

Removable front

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