Our standard anaerobic chamber with HEPA filtration.

Our standard anaerobic chamber with HEPA filtration.

Options and Specs Images and Video

Microbiology International can now offer the unique Whitley Internal HEPA Filtration System to provide a high degree of product/sample protection on Whitley Anaerobic Workstations. HEPA fitted workstations are ideal for use as part of a pharmaceutical manufacturing process, for the cultivation of slow-growing anaerobes, or for applications requiring complex manipulations under anaerobic conditions (ie biochemical assays).

Using innovative circulation technology, all the atmosphere in the chamber passes through the filter every four seconds, quickly creating and maintaining a particle-free environment (tested down to 0.3μm – exceeding ISO 14644 Class 3, as referenced in the Cell Tissue Culture Directive, or Class 1 of US Federal Standard 209E). Unlike some other systems, Microbiology International integrates the filter within the workstation.

To introduce samples or equipment, the built-in, 12 liter, rapid-cycle airlock has the capacity to introduce up to 40 Petri dishes into the workstation in 60 seconds. There is also the option to specify the 15cm letterbox, ideal for quickly transferring all sizes of dishes and small items.

Whatever your application, we can tailor the Whitley A35 Workstation to your requirements. For example, if you want to be able to use electrical equipment inside the chamber, you could include the option of a built-in, interior electrical socket. If you require the ability to thoroughly sanitize your workstation between experiments or need to introduce large items of equipment, you may want to specify the removable front option.

A35 Front Options

From a relatively small footprint we have been able to maximize incubation capacity to create the perfect environment, whether for a few plates or hundreds of plates.

The Whitley A35 Anaerobic Workstation is the latest innovation in workstation technology from Don Whitley Scientific. Complete with a built-in, rapid airlock and intuitive touch-screen control panel, this new workstation allows you to work completely glove and sleeve-free.

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HEPA Anaerobic Workstation


Rather than use ‘off-the-shelf’ filters, the Whitley HEPA Filtration System uses filters designed specifically for the product in conjunction with one of the world’s leading filter manufacturers.
Fitted with patented oval ports, equipped with sleeves and cuffs, which provide greater freedom of movement and operator comfort.
Automated humidity control with no user maintenance
Rapid, built-in airlock with mechanical and electrical interlocks - no risk of compromising conditions inside the chamber
Touch-screen interface with password protected user levels
Dual gas operation and automatic commissioning cycle
Constructed from 10mm thick annealed acrylic, most robust chambers available
Tangential fans create laminar flow in chamber, internal atmosphere is constantly being circulated over catalyst, Anotox, and heating mat for a consistent and strictly anaerobic environment
Double internal power socket, 2 Amp max
Accommodates up to 400 plates with ample work space, two porthole doors
Gas requirements: 1 x ANO2 = 10% H2, 10% CO2, 80% N2 -OR- 1 x ANO2 and Nitrogen
No requirement to bake catalyst, simply replace catalyst and Anotox annually
Available removable front that can be removed and replaced in 30 seconds without the use of tools. This allows for thorough cleaning or installation of larger equipment
The Whitley A35 is available with custom port options. The two ports can be either rapid entry or conventional sleeved ports by choosing the removable front option.
  • Atmospheric cleanliness
  • Exceeds international HEPA standards
  • Available on most Workstations

Eliminate contamination during extended growth

All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly. DWS filters are located inside the workstation, which prevents the filter becoming saturated with moisture and rendered ineffective. Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3.

HEPA Filtration

An Introduction to HEPA Filtration

HEPA Filtration

Tests were carried out in the DWS GLP compliant laboratories on the use of the Whitley HEPA Filtration System within an anaerobic chamber. Two Whitley Workstations were used - one fitted with HEPA filtration, one without HEPA. These tests demonstrated that the system produces a rapid and substantial reduction in bacterial contamination of the atmosphere. Atmoshpere was sampled from three locations - the chamber interior, the location adjacent to the pressure relief valve outlet, and the external atmosphere adjacent to the chamber (in the operator’s position). The results confirmed that no bacterial colonies were recovered from any of the sampling points after 2 minutes of the introduction of bacteria by nebulization.

Whitley HEPA Filtration System

Whitley HEPA Filtration System



Anaerobic Conditions
Monitoring System
This factory-fitted option monitors and displays real time oxygen levels within the workstation. Depending on the percentage of oxygen present, the chamber/airlock symbol on the touch screen will read green, yellow or red. The electronic Anaerobic Conditions Monitoring System is complete with oxygen sensor and a special software package. The oxygen sensor is located inside the chamber for accurate, real-time readings. The sensor is easily and quickly calibrated via the touch screen.

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Our custom-designed trolley frees up bench space and allows the chamber to be moved. The trolleys are built with high quality tubular steel and smooth rolling lockable wheels. They offer a sturdy and stable frame to support your use of our anaerobic and microaerophilic workstations.
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Gas Pressure Monitor
Designed as a method of monitoring the gas supply. The system will indicate the status of both sides of the switchover: FULL – IN SERVICE, and DEPLETED. When a cylinder or cylinder bank is depleted, the green light will change to a red light, and the audible alarm will sound.
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15cm Letterbox
Ideal for quickly introducing small quanitites of samples and supplies

Spare Cable Gland
Used to accommodate a power or data cable without affecting the chamber conditions

Data Logging
Access temperature, humidity, chamber pressure and anaerobic conditions. Stores up to 30 days of system information, accessed via USB port.

Internal Storage Trays
Maximum 2 per unit; positioned under the shelf

10 Plate Dish Rack
Easily stack and move 10 petri dishes. Prevents spills with a rigid design


Capacity (Liters) 300
Capacity (Petri Dishes) 400 - 600
Port/Airlock Capacity 40 x 90mm Petri dishes
Airlock Size 12 liters
Refrigeration Not available
HEPA Filtration Fitted as standard
Airlock Cycle Time 60 seconds
Automatic Dehumidifier Fitted as standard
Removable Front Option Available
Dimensions (w/d/h - mm) 1255 x 720 x 710
Weight (lbs/kg) 220 / 100
Temperature Range (°C) 10°C above ambient up to 45°C
Porthole System Manual Sleeved Ports

Image Gallery

A35 with HEPA Gallery
Click image to enlarge

Featured Videos

Whitley HEPA Filtration System

Whitley HEPA Filtration System

A35 Anaerobic Workstation

A35 Anaerobic Workstation Introduction

Dr Andrew Dempster, Centre for Biomolecular Sciences, University of Nottingham_Testimonial Video

Dr Andrew Dempster Testimonial

Dr Jane Freeman from Leeds General Infirmary

Dr Jane Freeman Testimonial

Whitley Anaerobic Workstations

Whitley Anaerobic Workstations

Anaerobic Conditions Monitor Animation

Anaerobic Conditions Monitor Animation

Removable Front

Removable front

Why Choose Whitley?

Why Choose Whitley?

Motorized Trolley