anaerobic workstation,

  • GMP Landing Page

    GMP capable isolator providing sterile laminar airflow under strict anaerobic conditions.

    GMP capable isolator providing sterile laminar airflow under strict anaerobic conditions.

     

    The A135 GMP and A155 GMP Workstations maintain strict anaerobic conditions, operating from two gas supplies for the most effective and efficient running costs. These anaerobic chambers exceed the requirements of Grade A air cleanliness (EU GMP) and ISO 14644-1 Class 3 both at rest and in operation. They combine sterile laminar airflow, positive operating pressure, and physical isolation, to provide highly effective product protection. Due to being a completely closed, re-circulating, isolator system, these workstations can be housed in a Grade D cleanroom during GMP compliant use.

    Our manufacturing partner of over 20 years, Don Whitley Scientific, has focused their efforts into developing a new workstation designed for anaerobic processing that can be operated in a GMP environment. This decision was based on many customer inquiries into our market leading HEPA workstations that required more precise air flow technology and software to meet GMP standards. Based on customer feedback and a desire to provide a cutting edge system that presents a new class of anaerobic workstations the A135 and A155 GMP Workstations were designed.

    These workstations are ideal for a range of applications such as fecal microbiota transplant (FMT), live biotherapeutics, manufacture of probiotics, biopharmaceuticals and microbiome therapeutics.

    An optional HEPA Filtered Airlock is also available, which includes the incorporation of a H14 HEPA filter to ensure 100% reduction in airborne particulate in the airlock over the course of the cycle. This ensures Grade A particulate counts in the main chamber are not compromised.

    Each workstation is tested to ensure compliance with the relevant portions of ISO 14644-3. In addition, tests carried out in our own laboratories confirm the integral HEPA filtered laminar flow system meets the requirements set out in BS EN 12469:2000 with regard to protection of the working area from particulate and microbial contamination.

     

     

    Applications include therapeutic products requiring controlled atmospheric conditions (anaerobic/hypoxic) for manufacture:

     
     

    GMP range of workstations are validated to be 99.9997% efficient in a single pass with an H14 filter.

    This is the only product providing atmospheric condition control and unidirectional flow.

    Chamber

    Laminar Flow HEPA Filtration

    Operational particulate counts are kept low even when large amounts of particulate is generated.

    Benefits of a GMP Isolator Workstation

    Clean Room: Cascade Approach

    Traditionally ‘cascade’ from class D - >A.  This adds significant cost to any project requiring the build-out of several areas for different air classification environments. 

    airflow

    Isolator: All-In-One Approach

    The air classification required for the background environment depends on design of the isolator and its application. It should be controlled and for aseptic processing it should be at least grade D.

    airflow

     

    Two Workstation Size Options

    Whitley A135 GMP

    Accommodates a single user with single transfer airlock. Strict anaerobic conditions in sterile laminar flow environment.

    airflow

    Specs:

    Capacity (Liters) 900
    HEPA Fitted as standard
    Gas Supply ANO2/N2
    Airlock Cycle Time 120 seconds
    Airlock capacity: 10 x 500ml Duran bottles
    Airlock Size (liters) 17.50
    Dimensions (w/d/h in mm): 1500 x 1130 x 1080
    Weight (lbs/kg): 649/295
    Temperature range 4ºC above ambient up to 45ºC
    Electrical 110 V, 50-60 Hz

    Whitley A155 GMP

    Accommodates two user with double transfer airlocks. Strict anaerobic conditions in sterile laminar flow environment.

    airflow

    Specs:

    Capacity (Liters) 1800
    HEPA Fitted as standard
    Gas Supply ANO2/N2
    Airlock Cycle Time 120 seconds
    Airlock capacity 10 x 500ml Duran bottles in each airlock
    Airlock Size (liters) 17.50
    Dimensions (w/d/h in mm): 2940 x 1130 x 1080
    Weight (lbs/kg): 1298/590
    Temperature range 4ºC above ambient up to 45ºC
     

    Related Products

     
  • Whitley Incubation Box

    Whitley Incubation Box
    Whitley Incubation Box
    Click image to enlarge

    New from Don Whitley Scientific is the rectangular, transparent and stackable anaerobic ‘jar’. It can be used to create the perfect environmental conditions for the isolation and cultivation of anaerobic and microaerobic organisms, and for cell culture applications.

    Whitley Incubation Boxes were designed specifically to be stacked on top of each other inside an incubator or on the bench, saving valuable space. The main box (without the lid in place and with no samples inside) can be nested with other empty boxes so that work in progress takes up minimal space.

    Whitley Incubation Box will accommodate either 12-14 x 90mm Petri dishes or 5 x standard multi-well plates. The four latches used to open and close the box are attached to the lid so there are no loose parts to misplace.

    The Whitley Incubation Box may be used to transfer items into and out of a Whitley Workstation. Whitley Incubation Box has been designed to fit through the 35-sized airlock and every larger Whitley airlock. This application is ideal when you want to move samples from one workstation to another and when you want to preserve your samples in modified atmospheric conditions while they continue to grow in an external incubator.

    The design of the Whitley Incubation Box includes a safety valve, which ensures an appropriate level of vacuum is achieved without damaging the box itself. It is durable and lightweight and can be used to create multiple atmospheres.

     

    Features

    • Accommodates both Petri dishes and multi-well plates.
    • Both lids and bases can be nested for storage.
    • Flexibility to create different atmospheric conditions.
    • Latches are permanently attached to the lid so there are no loose parts to misplace.
    • Completed boxes can be stacked on top of each other for incubation.
    • Rectangular shape saves space in the incubator.
    • Includes a safety valve to ensure an appropriate level of vacuum is achieved without damaging the box itself.
    • Durable and lightweight.

    Specs

    Capacity Petri Dishes 2-14 x 90mm Petri Dishes or 5 x standard multi-well plates
    Gas Supplies Whitley Jar Gassing System or gas generating envelopes
    Dimensions (W/D/H mm) 235 / 164 / 130

     
  • Whitley Workstation A135 GMP

    GMP capable isolator providing sterile laminar airflow under strict anaerobic conditions.

    GMP capable isolator providing sterile laminar airflow under strict anaerobic conditions.

    Options and Specs

    Microbiology International as you know, provides the most advanced anaerobic workstations on the market with many standard features such as yearlong catalyst life without baking, maintenance free humidity removal and touchscreen controls. Additional options include class leading HEPA filtration (better than a Class III environment according to ISO 14644-3), HEPA exhaust filtration to decrease 99.999% potential pathogen escape, and our patented Anaerobic Conditions Monitoring System (ACMS) which includes a daily catalyst monitor.

    Our manufacturing partner of over 20 years, Don Whitley Scientific, have focused their efforts into developing a new workstation designed for anaerobic processing that can be operated in a GMP environment. This decision was based on many customer inquiries into our market leading HEPA workstations that required more precise air flow technology and software to meet GMP standards. Based on customer feedback and a desire to provide a cutting edge system that presents a new class of anaerobic workstations the A135 GMP Workstation was designed.

    The A135 GMP Workstation* is the latest workstation to be added to the anaerobic range, and is specifically designed to be used as a clean-air isolator in processes following Good Manufacturing Practice (GMP). This new anaerobic workstation provides Grade A air cleanliness (EU GMP), and combines sterile laminar airflow, positive operating pressure, and physical isolation, to provide highly effective product protection. Due to being a completely closed, re-circulating, isolator system, this workstation can be housed in a Grade D cleanroom during GMP compliant use.

    An optional HEPA Filtered Airlock is also available, which includes the incorporation of a H14 HEPA filter to ensure 100% reduction in airborne particulate in the airlock over the course of the cycle. This ensures Grade A particulate counts in the main chamber are not compromised.

    Each workstation is tested by an independent organisation to ensure compliance with ISO 14644-3. In addition, tests carried out in our own laboratories confirm the integral HEPA filtered laminar flow system meets the requirements set out in BS EN 12469:2000 with regard to protection of the working area from particulate and microbial contamination.

    Download Brochure

    Image Gallery

    A135LF Gallery
    Click image to enlarge

    Features:

    A color, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
    Automated humidity control with no user maintenance
    The GMP Workstation exceeds the requirements of Grade A air cleanliness (EU GMP) and ISO 14644-1 Class 3 both at rest and in operation.
    Rapid, built-in transfer airlock
    Internal storage shelf
    Dual gas operation and automatic commissioning cycle
    Constructed from 10mm thick annealed acrylic, most robust chambers available
    Tangential fans create laminar flow in chamber, internal atmosphere is constantly being circulated over catalyst, Anotox, and heating mat for a consistent and strictly anaerobic environment
    A135: 560 liter work space capacity
    No requirement to bake catalyst, simply replace catalyst and Anotox annually
    Chamber can be used for microaerophilic incubation, simply remove Anotox and catalyst and connect pre-mixed gas tank
    Touch screen interface to control all chamber settings, can be password protected
    Double internal power socket, 2 Amp max
     

    Options:


    Trolley
    Our custom-designed trolley frees up bench space and allows the chamber to be moved. The trolleys are built with high quality tubular steel and smooth rolling lockable wheels. They offer a sturdy and stable frame to support your use of our anaerobic and microaerophilic workstations.
    See Details

    15cm Letterbox
    Ideal for quickly introducing small quanitites of samples and supplies

    Spare Cable Gland
    Used to accommodate a power or data cable without affecting the chamber conditions

    Data Logging
    Access temperature, humidity, chamber pressure and anaerobic conditions. Stores up to 30 days of system information, accessed via USB port.

    Internal Storage Trays
    Maximum 2 per unit

    Whitley Petrifoto
    Imaging System
    Conveniently fits inside all Whitley Workstations and allows users to capture sample photographs during incubation

    Anaerobic Conditions
    Monitoring System
    Monitors and displays real time oxygen levels within the workstation. Depending on the percentage of oxygen present, the chamber/airlock symbol on the touch screen will read green, yellow or red. The system is complete with oxygen sensor and a special software package. The oxygen sensor is located inside the and quickly calibrated via the touch screen

    Integrated Touch
    Screen Computer
    System
    For easy data entry the touch screen is conveniently mounted inside the chamber for ergonomic use and connects to an external computer

    Specs:

    Capacity (Liters) 900
    HEPA Fitted as standard
    Gas Supply ANO2/N2
    Airlock Cycle Time 120 seconds
    Airlock capacity: 10 x 500ml Duran bottles
    Airlock Size (liters) 17.50
    Dimensions (w/d/h in mm): 1500 x 1130 x 1080
    Weight (lbs/kg): 649/295
    Temperature range 4ºC above ambient up to 45ºC
    Electrical 110 V, 50-60 Hz

    Featured Videos

    Dr Andrew Dempster, Centre for Biomolecular Sciences, University of Nottingham_Testimonial Video

    Dr Andrew Dempster Testimonial

    Dr Jane Freeman from Leeds General Infirmary

    Dr Jane Freeman Testimonial

    Whitley Anaerobic Workstations

    Whitley Anaerobic Workstations

    Anaerobic Conditions Monitor Animation

    Anaerobic Conditions Monitor Animation

    Microbiology Research at the School of Dentistry, University of Leeds

    Microbiology Research at the School of Dentistry, University of Leeds

    Why Choose Whitley?

    Why Choose Whitley?


    Motorized Trolley

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Removable Front

    Whitley Workstation - Removable Front

    Whitley Workstation - O2 Cell Calibration

    Whitley Workstation - O2 Cell Calibration

  • Whitley Workstation A135 HEPA

    Our standard anaerobic chamber with HEPA filtration.

    Our standard anaerobic chamber with HEPA filtration.

    Options and Specs

    The Whitley A135 Anaerobic Workstation is the latest innovation in workstation technology from Don Whitley Scientific. The tallest, deepest, widest anaerobic workstation in our extensive range. It is specifically designed to maintain strict anaerobic conditions within a controlled environment. It provides excellent conditions for the processing, incubation and examination of samples without exposure to atmospheric oxygen. With a usable internal volume of 560 liters, a variety of equipment can be accommodated within the chamber. As well as providing ideal conditions for anaerobic studies, this workstation operates from two gas supplies for the most cost-effective running conditions.

    The A135 has built-in rapid airlock. This ensures samples can be transferred into the workstation atmosphere as soon as possible (takes only 60 seconds).

    Can be fitted with the optional Whitley Internal HEPA Filtration System. All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly. DWS filters are located inside the workstation, which prevents the filter becoming saturated with moisture and rendered ineffective. Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3.

    The A135 is fitted with a removable front. The removable front facilitates thorough cleaning, easy replacement of the HEPA filter, and the transfer of bulk samples and larger pieces of equipment for use in the workstation.

    The internal atmosphere is circulated continuously - contributing to even temperature distribution and ensuring any internal particulates will be in motion before capture by the filter.

    Fully integrated gas control avoids bulky add-on systems - reduces the need for additional bench space.

    Download Brochure

    Image Gallery

    A135 Gallery
    Click image to enlarge

    Features:

    A color, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status.
    Automated humidity control with no user maintenance
    Rapid, built-in transfer airlock
    Internal storage shelf
    Dual gas operation and automatic commissioning cycle
    Constructed from 10mm thick annealed acrylic, most robust chambers available
    Tangential fans create laminar flow in chamber, internal atmosphere is constantly being circulated over catalyst, Anotox, and heating mat for a consistent and strictly anaerobic environment
    A135: 560 liter work space capacity
    No requirement to bake catalyst, simply replace catalyst and Anotox annually
    Chamber can be used for microaerophilic incubation, simply remove Anotox and catalyst and connect pre-mixed gas tank
    Touch screen interface to control all chamber settings, can be password protected
    Double internal power socket, 2 Amp max
     

    Options:

    HEPA filtration for
    sample protection
    The chamber atmosphere passes through the filter every four seconds, quickly creating and maintaining a particle-free environment (tested down to 0.3μm, exceeding ISO 14644 Class 3, as referenced in the Cell Tissue Culture Directive, or Class 1 of US Federal Standard 209E). Clean-up time after a period of activity is considerably less than the 20 minutes allowed by the standard.

    Trolley
    Our custom-designed trolley frees up bench space and allows the chamber to be moved. The trolleys are built with high quality tubular steel and smooth rolling lockable wheels. They offer a sturdy and stable frame to support your use of our anaerobic and microaerophilic workstations.
    See Details

    15cm Letterbox
    Ideal for quickly introducing small quanitites of samples and supplies

    Spare Cable Gland
    Used to accommodate a power or data cable without affecting the chamber conditions

    Data Logging
    Access temperature, humidity, chamber pressure and anaerobic conditions. Stores up to 30 days of system information, accessed via USB port.

    Internal Storage Trays
    Maximum 2 per unit

    Whitley Petrifoto
    Imaging System
    Conveniently fits inside all Whitley Workstations and allows users to capture sample photographs during incubation

    Anaerobic Conditions
    Monitoring System
    Monitors and displays real time oxygen levels within the workstation. Depending on the percentage of oxygen present, the chamber/airlock symbol on the touch screen will read green, yellow or red. The system is complete with oxygen sensor and a special software package. The oxygen sensor is located inside the and quickly calibrated via the touch screen

    Integrated Touch
    Screen Computer
    System
    For easy data entry the touch screen is conveniently mounted inside the chamber for ergonomic use and connects to an external computer

    Specs:

    Capacity (Liters) 950
    HEPA Fitted as standard
    Gas Supply ANO2/N2
    Airlock Cycle Time 60 seconds
    Airlock capacity (Petri dishes): 40 plates
    Dimensions (w/d/h in mm): 1452 x 1056 x 1063
    Weight (lbs/kg): 386/175
    Temperature range 4ºC above ambient up to 45ºC
    Electrical 110 V, 50-60 Hz

    Featured Videos

    Whitley HEPA Filtration System

    Whitley HEPA Filtration System

    A35 Anaerobic Workstation

    A35 Anaerobic Workstation Introduction

    Dr Andrew Dempster, Centre for Biomolecular Sciences, University of Nottingham_Testimonial Video

    Dr Andrew Dempster Testimonial

    Dr Jane Freeman from Leeds General Infirmary

    Dr Jane Freeman Testimonial

    Whitley Anaerobic Workstations

    Whitley Anaerobic Workstations

    Anaerobic Conditions Monitor Animation

    Anaerobic Conditions Monitor Animation

    Microbiology Research at the School of Dentistry, University of Leeds

    Microbiology Research at the School of Dentistry, University of Leeds

    Why Choose Whitley?

    Why Choose Whitley?


    Motorized Trolley

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Removable Front

    Whitley Workstation - Removable Front

    Whitley Workstation - O2 Cell Calibration

    Whitley Workstation - O2 Cell Calibration

  • Whitley Workstation A155 HEPA

    Our standard anaerobic chamber with HEPA filtration.

    Our standard anaerobic chamber with HEPA filtration.

    Options and Specs
    Standard Anaerobic Workstation with HEPA filtration

    The Whitley A155 HEPA Workstation provides the ability to operate a variety of laboratory equipment or perform specialist tasks in a strictly controlled anaerobic environment. This workstation has a capacity of 1,900 litres; two airlocks; and two removable fronts.

    The huge capacity of this workstation means that it can accommodate a variety of laboratory equipment, eg cell sorters, colony pikers, high throughput bio-reactors, microscopes and spiral platers such as the Workstation WASP Touch.

    The A155 HEPA is equipped with the Whitley Internal HEPA Filtration System, which reduces particle counts inside the chamber to levels exceeding the requirements of ISO 14644 Class 3.


    Image Gallery

    A135 Gallery
    Click image to enlarge

    Features:

    A color, touch-screen control panel for ease of use and for visual display of parameters such as temperature, humidity, and airlock cycle status
    Two deep airlocks, one at either end of the workstation, that can be operated independently and take only 60 seconds to run a cycle. Each airlock can accommodate 10 x 500ml Duran bottles
    Two removable fronts to enable the transfer of larger batches of samples or pieces of equipment for use inside the chamber
    Patented oval ports enable you to work comfortably, gloved or bare handed. Each port includes an isolator switch so that you can use one port at a time, if required. The fact that there are two sets of ports means that two researchers can work in the chamber side-by-side
    Two new options are available: a rotating turntable (up to 3 per shelf) to hold up to 80 x 90mm Petri dishes; and the movable platform to facilitate the movement of heavy items of equipment inside the chamber.
    Double internal power socket, 2 Amp max
    Shelves with optional storage trays
     

    Options:

    HEPA filtration for
    sample protection
    The chamber atmosphere passes through the filter every four seconds, quickly creating and maintaining a particle-free environment (tested down to 0.3μm, exceeding ISO 14644 Class 3, as referenced in the Cell Tissue Culture Directive, or Class 1 of US Federal Standard 209E). Clean-up time after a period of activity is considerably less than the 20 minutes allowed by the standard.

    Trolley
    Our custom-designed trolley frees up bench space and allows the chamber to be moved. The trolleys are built with high quality tubular steel and smooth rolling lockable wheels. They offer a sturdy and stable frame to support your use of our anaerobic and microaerophilic workstations.
    See Details

    Front Loading 9cm Letterbox
    A single sample entry system is ideal for quickly introducing culture flasks, Petri dishes and other small items

    Spare Cable Gland
    Used to accommodate a power or data cable without affecting the chamber conditions

    Data Logging
    Access temperature, humidity, chamber pressure and anaerobic conditions. Stores up to 30 days of system information, accessed via USB port.

    Anaerobic Conditions
    Monitoring System
    Monitors and displays real time oxygen levels within the workstation. Depending on the percentage of oxygen present, the chamber/airlock symbol on the touch screen will read green, yellow or red. The system is complete with oxygen sensor and a special software package. The oxygen sensor is located inside the and quickly calibrated via the touch screen

    Integrated Touch
    Screen Computer
    System
    For easy data entry the touch screen is conveniently mounted inside the chamber for ergonomic use and connects to an external computer

    Spotlight
    The Whitley LED Spotlight option provides directional lighting to assist in the early identification of sample growth or other specimen examination.

    Electronic gas
    leak detector
    The Electronic Gas Leak Detector provides an audible tick and flashing LED when exposed to H2 gas at 50 ppm or more.

    Conventional Porthole
    System
    These sleeved ports provide considerable freedom of movement and comfort when working in the chamber for long periods.

    Vacuum Take-off
    Port
    A Vacuum Take-off Port provides a method of aspirating used liquid media from inside the workstation in safety and without the risk of spillage or contamination.

    Specs:

    Capacity (Liters) 1900
    HEPA Fitted as standard
    Gas Supply ANO2/N2
    Airlock Cycle Time 60 seconds
    Airlock capacity: 16 Liters
    Dimensions (w/d/h in mm): 2875x1056x993
    Weight (lbs/kg): 771/350
    Temperature range 4ºC above ambient up to 45ºC
    Electrical 110 V, 50-60 Hz

    Featured Videos

    Whitley HEPA Filtration System

    Whitley HEPA Filtration System

    A35 Anaerobic Workstation

    A35 Anaerobic Workstation Introduction

    Dr Andrew Dempster, Centre for Biomolecular Sciences, University of Nottingham_Testimonial Video

    Dr Andrew Dempster Testimonial

    Dr Jane Freeman from Leeds General Infirmary

    Dr Jane Freeman Testimonial

    Whitley Anaerobic Workstations

    Whitley Anaerobic Workstations

    Anaerobic Conditions Monitor Animation

    Anaerobic Conditions Monitor Animation

    Microbiology Research at the School of Dentistry, University of Leeds

    Microbiology Research at the School of Dentistry, University of Leeds

    Why Choose Whitley?

    Why Choose Whitley?


    Motorized Trolley

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Removable Front

    Whitley Workstation - Removable Front

    Whitley Workstation - O2 Cell Calibration

    Whitley Workstation - O2 Cell Calibration

  • Whitley Workstation A35 HEPA

    Our standard anaerobic chamber with HEPA filtration.

    Our standard anaerobic chamber with HEPA filtration.

    Options and Specs Images and Video

    Microbiology International can now offer the unique Whitley Internal HEPA Filtration System to provide a high degree of product/sample protection on Whitley Anaerobic Workstations. HEPA fitted workstations are ideal for use as part of a pharmaceutical manufacturing process, for the cultivation of slow-growing anaerobes, or for applications requiring complex manipulations under anaerobic conditions (ie biochemical assays).

    Using innovative circulation technology, all the atmosphere in the chamber passes through the filter every four seconds, quickly creating and maintaining a particle-free environment (tested down to 0.3μm – exceeding ISO 14644 Class 3, as referenced in the Cell Tissue Culture Directive, or Class 1 of US Federal Standard 209E). Unlike some other systems, Microbiology International integrates the filter within the workstation.

    To introduce samples or equipment, the built-in, 12 liter, rapid-cycle airlock has the capacity to introduce up to 40 Petri dishes into the workstation in 60 seconds. There is also the option to specify the 15cm letterbox, ideal for quickly transferring all sizes of dishes and small items.

    Whatever your application, we can tailor the Whitley A35 Workstation to your requirements. For example, if you want to be able to use electrical equipment inside the chamber, you could include the option of a built-in, interior electrical socket. If you require the ability to thoroughly sanitize your workstation between experiments or need to introduce large items of equipment, you may want to specify the removable front option.

    A35 Front Options

    From a relatively small footprint we have been able to maximize incubation capacity to create the perfect environment, whether for a few plates or hundreds of plates.

    The Whitley A35 Anaerobic Workstation is the latest innovation in workstation technology from Don Whitley Scientific. Complete with a built-in, rapid airlock and intuitive touch-screen control panel, this new workstation allows you to work completely glove and sleeve-free.

    Download Brochure
    HEPA Anaerobic Workstation

    Features:

    Rather than use ‘off-the-shelf’ filters, the Whitley HEPA Filtration System uses filters designed specifically for the product in conjunction with one of the world’s leading filter manufacturers.
    Fitted with patented oval ports, equipped with sleeves and cuffs, which provide greater freedom of movement and operator comfort.
    Automated humidity control with no user maintenance
    Rapid, built-in airlock with mechanical and electrical interlocks - no risk of compromising conditions inside the chamber
    Touch-screen interface with password protected user levels
    Dual gas operation and automatic commissioning cycle
    Constructed from 10mm thick annealed acrylic, most robust chambers available
    Tangential fans create laminar flow in chamber, internal atmosphere is constantly being circulated over catalyst, Anotox, and heating mat for a consistent and strictly anaerobic environment
    Double internal power socket, 2 Amp max
    Accommodates up to 400 plates with ample work space, two porthole doors
    Gas requirements: 1 x ANO2 = 10% H2, 10% CO2, 80% N2 -OR- 1 x ANO2 and Nitrogen
    No requirement to bake catalyst, simply replace catalyst and Anotox annually
    Available removable front that can be removed and replaced in 30 seconds without the use of tools. This allows for thorough cleaning or installation of larger equipment
    The Whitley A35 is available with custom port options. The two ports can be either rapid entry or conventional sleeved ports by choosing the removable front option.
    • Atmospheric cleanliness
    • Exceeds international HEPA standards
    • Available on most Workstations

    Eliminate contamination during extended growth

    All the atmosphere passes through the filter hundreds of times an hour, which ensures the chamber environment is cleaned quickly. DWS filters are located inside the workstation, which prevents the filter becoming saturated with moisture and rendered ineffective. Levels of atmospheric cleanliness inside the workstation exceed the requirements of ISO 14644 Class 3.

    HEPA Filtration

    An Introduction to HEPA Filtration

    HEPA Filtration

    Tests were carried out in the DWS GLP compliant laboratories on the use of the Whitley HEPA Filtration System within an anaerobic chamber. Two Whitley Workstations were used - one fitted with HEPA filtration, one without HEPA. These tests demonstrated that the system produces a rapid and substantial reduction in bacterial contamination of the atmosphere. Atmoshpere was sampled from three locations - the chamber interior, the location adjacent to the pressure relief valve outlet, and the external atmosphere adjacent to the chamber (in the operator’s position). The results confirmed that no bacterial colonies were recovered from any of the sampling points after 2 minutes of the introduction of bacteria by nebulization.

    Whitley HEPA Filtration System

    Whitley HEPA Filtration System

     

    Options:

    Anaerobic Conditions
    Monitoring System
    This factory-fitted option monitors and displays real time oxygen levels within the workstation. Depending on the percentage of oxygen present, the chamber/airlock symbol on the touch screen will read green, yellow or red. The electronic Anaerobic Conditions Monitoring System is complete with oxygen sensor and a special software package. The oxygen sensor is located inside the chamber for accurate, real-time readings. The sensor is easily and quickly calibrated via the touch screen.

    See Details


    Trolley
    Our custom-designed trolley frees up bench space and allows the chamber to be moved. The trolleys are built with high quality tubular steel and smooth rolling lockable wheels. They offer a sturdy and stable frame to support your use of our anaerobic and microaerophilic workstations.
    See Details

    Gas Pressure Monitor
    Designed as a method of monitoring the gas supply. The system will indicate the status of both sides of the switchover: FULL – IN SERVICE, and DEPLETED. When a cylinder or cylinder bank is depleted, the green light will change to a red light, and the audible alarm will sound.
    See Details

    15cm Letterbox
    Ideal for quickly introducing small quanitites of samples and supplies

    Spare Cable Gland
    Used to accommodate a power or data cable without affecting the chamber conditions

    Data Logging
    Access temperature, humidity, chamber pressure and anaerobic conditions. Stores up to 30 days of system information, accessed via USB port.

    Internal Storage Trays
    Maximum 2 per unit; positioned under the shelf

    10 Plate Dish Rack
    Easily stack and move 10 petri dishes. Prevents spills with a rigid design

    Specs:

    Capacity (Liters) 300
    Capacity (Petri Dishes) 400 - 600
    Port/Airlock Capacity 40 x 90mm Petri dishes
    Airlock Size 12 liters
    Refrigeration Not available
    HEPA Filtration Fitted as standard
    Airlock Cycle Time 60 seconds
    Automatic Dehumidifier Fitted as standard
    Removable Front Option Available
    Dimensions (w/d/h - mm) 1255 x 720 x 710
    Weight (lbs/kg) 220 / 100
    Temperature Range (°C) 10°C above ambient up to 45°C
    Porthole System Manual Sleeved Ports

    Image Gallery

    A35 with HEPA Gallery
    Click image to enlarge

    Featured Videos

    Whitley HEPA Filtration System

    Whitley HEPA Filtration System

    A35 Anaerobic Workstation

    A35 Anaerobic Workstation Introduction

    Dr Andrew Dempster, Centre for Biomolecular Sciences, University of Nottingham_Testimonial Video

    Dr Andrew Dempster Testimonial

    Dr Jane Freeman from Leeds General Infirmary

    Dr Jane Freeman Testimonial

    Whitley Anaerobic Workstations

    Whitley Anaerobic Workstations

    Anaerobic Conditions Monitor Animation

    Anaerobic Conditions Monitor Animation

    Microbiology Research at the School of Dentistry, University of Leeds

    Microbiology Research at the School of Dentistry, University of Leeds

    Why Choose Whitley?

    Why Choose Whitley?


    Motorized Trolley

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Airlock Operation

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeve Assembly

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Sleeved Port Operation

    Whitley Workstation - Removable Front

    Whitley Workstation - Removable Front

    Whitley Workstation - O2 Cell Calibration

    Whitley Workstation - O2 Cell Calibration